Tissue culture is a ticklish propagation technique, giving nursery growers one more way to make plants quickly, accurately and in great numbers.

When we were introduced to plant propagation back in Grade 6, we learned the three primary methods of producing new plants are by seed, stem cuttings and division. We also touched on grafting and air layering.

For today’s Grade 6 biology pupils, we would add tissue culture to the list.

Tissue culture is a ticklish propagation technique, giving nursery growers one more way to make plants quickly, accurately and in great numbers.

Also called micro propagation, tissue culture is a technique of making new plants in a lab under sterile conditions. Only a few cells are required in the process. As few as one or two leaves or roots of a parent plant can produce hundreds of offspring.

So far, tissue culture has been found to be easier using fleshy or herbaceous plants rather than tough, woody plants. Two of the easiest plants to reproduce by tissue culture are African violets and orchids. A limited number of herbs are now produced, and it is expected that soon, many annuals, perennials, herbs and indoor plants will be grown by tissue culture.

Want to try tissue culture at home? You’ll need a face mask, bleach, rubbing alcohol, aluminum foil, petri dishes with lids, dish soap and tweezers. You’ll also need a recipe for culture medium made up of several ingredients, or available as a prepared mixture.

Sterilize the work area by thoroughly cleaning your hands, tools and containers. Find a leaf or two and use a razor blade to chop the leaf into as many tiny pieces as you like. Prepare the mixture according to directions and add the plant tissue. Cover and maintain plant sterility.

Move the dish to a location with artificial light and temperature of 25 C. Provide light for 18 hours a day.

In short order, each tiny plant segment should grow. Once seedlings are large enough to handle and separate, plant in small pots with a soilless growing medium and watch them grow. If fungus forms during the process, discard everything and start again.

It may take decades before researchers find ways to make tissue culture a common plant production practice.

It already has been found that orchids are much easier to grow by tissue culture.

In the past, orchids were propagated by division, a slow, expensive process. Today, thanks to tissue culture, it’s quick and efficient, evidenced by their availability at every nursery, grocer and big-box store.

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QOSHE - GARDEN CLIPPINGS: Tissue culture can make new plants quickly - John Degroot
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GARDEN CLIPPINGS: Tissue culture can make new plants quickly

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19.01.2024

Tissue culture is a ticklish propagation technique, giving nursery growers one more way to make plants quickly, accurately and in great numbers.

When we were introduced to plant propagation back in Grade 6, we learned the three primary methods of producing new plants are by seed, stem cuttings and division. We also touched on grafting and air layering.

For today’s Grade 6 biology pupils, we would add tissue culture to the list.

Tissue culture is a ticklish propagation technique, giving nursery growers one more way to make plants quickly, accurately and in great numbers.

Also called micro propagation, tissue culture is a technique of making new plants in a lab under sterile conditions. Only a few cells are required in the process. As few as one or two leaves........

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